Application

Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free is an antibody against APC for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human kidney and cardiac muscle.
Immunocytochemistry Analysis: A representative detected an increased APC expression in human eyelid adipose-derived stem cells (hEASCs) after neuroinduction at passage 8 (Zhou, J., et al. (2014). J. Cell. Mol. Med. 18(2):326-343).
Immunofluorescence Analysis: A representative lot detected a granular APC staining pattern at a subapical region of epithelial cells in fallopian mucosa by fluorescent immunohistochemistry staining of paraffin-embedded, paraformaldehyd-fixed fallopian tube tissue sections (Kessler, M., et al. (2012). Am. J. Pathol. 180(1):186-198).

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Adenomatous polyposis coli protein (UniProt P25054; also known as Deleted in polyposis 2.5, Protein APC) is encoded by the APC (also known as DP2.5) gene (Gene ID 324) in human. The multifunctional tumor suppressor APC is a large scaffold protein that contains multiple armadillo repeat (ARM) domains (a.a. 453-767 of human APC spliced isoform 1) for mediating various cellular functions, including Wnt signal regulation, cell adhesion, and chromosomal segregation during mitosis. APC, AXIN, casein kinase 1 (CK1) and glycogen synthase kinase 3 (GSK3) constitute the core proteins of the β-catenin destruction complex that functions as the negative regulator against Wnt signaling transduction. Mice with Apc gene mutations develop adenomas in the intestine due to Wnt hyperactivation, and functional loss of APC due to gene mutations is the hallmark of human colorectal cancers (CRC). APC also directs cholinergic synapse assembly between neurons and is commonly employed as a marker for monitoring stem cells neuronal differentiation.

Immunogen

Epitope: C-terminal region.

Human APC C-terminal fusion with MBP (maltose binding protein).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human cerebral cortex tissue.

Specificity

Recognizes human APC, molecular weight is approximately 310 kDa.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

311.6 kDa calculated.